Table 1. Primer sequences for semi-quantitative RT-PCR analysis.
mRNA | Oligonucleotide | |
---|---|---|
GAPDH | Forward | 5’- GGGCTGCTTTTAACTCTGGT-3’ |
Reverse | 5’- TGGCAGGTTTTTCTAGACGG-3’ | |
ITGA5 | Forward | 5’- GCAAGAGCCGGATAGAGGAC-3’ |
Reverse | 5’-GGACTGTAAACCGAAGGCCA-3’ | |
ITGB3 | Forward | 5’-CTGCCGTGACGAGATTGAGT-3’ |
Reverse | 5’-ATTAAGTGCCCCGGTACGTG-3’ |
GAPDH was designed according to Tuli et al. [30]. ITGA5 and ITGB3 were designed in our laboratory.