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. 2014 Nov 21;1:15. doi: 10.1186/s40634-014-0015-8

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© Gorth et al.; licensee Springer. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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In vivo delivery and localization microspheres. A. Delivery of microspheres to the rat NP from a custom 33-gauge needle under fluoroscopic guidance (lateral aspect). B. Higher magnification view of A. C. Differential interference contrast (DIC), axial image of entire disc 1 day post injection, showing NP and AF regions. D. Higher magnification view of the AF (inset from C.) showing microspheres (red). E. Microspheres (red, overlaying DIC image) embedded in the inner lamellae of the AF. F. Differential interference contrast, axial image of entire disc 14 days post injection, showing NP and AF regions. G. Higher magnification view (inset from F.) showing microspheres (red). H. Microspheres (red, overlaying DIC image), embedded in the NP.