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. 2015 Aug 20;15:168. doi: 10.1186/s12866-015-0507-4

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© Wen et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Reactivity of the GST-fusion proteins with the MAb D9. Western blots were performed to test the reactivity of MAb D9 with the GST-fusion proteins containing wild-type and truncated (a) or mutant (b) motifs derived from the motif WNDNTVRVS. Marker: PageRuler™ Prestained Protein Ladder. GST-7wt: fusion protein containing the motif WNDNTVRVS, GST-wtΔS: WNDNTVRV, GST-wtΔVS: WNDNTVR, GST-wtΔRVS: WNDNTV, GST-wtΔVRVS: WNDNT, GST-wtΔTVRVS: WNDN, GST-wtΔW-VRVS: NDNT, GST-T186S: WNDNS, GST-N183S: WSDNT, GST-D184G: WNGNT and GST-D184N: WNNNT