Table 3.
The oligonucleotides coding for wild type and truncated or mutant motifs
| Name | The sequences of oligonucleotides | Coding motifs |
|---|---|---|
| GST-wt-F | 5'-GATCCTGGAATGATAACACAGTTCGAGTCTCTTAA C-3' | WNDNTVRVS |
| GST-wt-R | 5'-TCGAG TTAAGAGACTCGAACTGTGTTATCATTCCAG-3' | |
| GST-wtΔS-F | 5'-GATCCTGGAATGATAACACAGTTCGAGTCTAA C-3' | WNDNTVRV |
| GST-wtΔS-R | 5'-TCGAG TTAGACTCGAACTGTGTTATCATTCCAG-3' | |
| GST-wtΔVS-F | 5'-GATCCTGGAATGATAACACAGTTCGATAA C-3' | WNDNTVR |
| GST-wtΔVS-R | 5'-TCGAG TTATCGAACTGTGTTATCATTCCAG-3' | |
| GST-wtΔRVS-F | 5'-GATCCTGGAATGATAACACAGTTTAA C-3' | WNDNTV |
| GST-wtΔRVS-R | 5'-TCGAG TTAAACTGTGTTATCATTCCAG-3' | |
| GST-wtΔVRVS-F | 5'-GATCCTGGAATGATAACACATAA C-3' | WNDNT |
| GST-wtΔVRVS-R | 5'-TCGAG TTATGTGTTATCATTCCAG-3' | |
| GST-wtΔTVRVS-F | 5'-GATCCTGGAATGATAACTAA C-3' | WNDN |
| GST-wtΔTVRVS-R | 5'-TCGAG TTAGTTATCATTCCAG-3' | |
| GST-wtΔW-VRVS-F | 5'-GATCCAATGATAACACATAA C-3' | NDNT |
| GST-wtΔW-VRVS-R | 5'-TCGAG TTATGTGTTATCATTG-3' | |
| GST-N183S-F | 5'-GATCCTGGTCTGATAACACATAA C-3' | WSDNT |
| GST-N183S-R | 5'-TCGAG TTATGTGTTATCAGACCAG-3' | |
| GST-D184G-F | 5'-GATCCTGGAATGGAAACACATAA C-3' | WDGNT |
| GST-D184G-R | 5'-TCGAG TTATGTGTTTCCATTCCAG-3' | |
| GST-D184N-F | 5'-GATCCTGGAATAATAACACATAA C-3' | WNNNT |
| GST-D184N-R | 5'-TCGAG TTATGTGTTATTATTCCAG-3' | |
| GST-T186S-F | 5'-GATCCTGGAATGATAACTCATAA C-3' | WNDNS |
| GST-T186S-R | 5'-TCGAG TTATGAGTTATCATTCCAG-3' |
Note: All primers expressing GST fusion proteins carried the BamHI restriction endonuclease site upstream and the XhoI site downstream, which are shown in italics. The stop codons are shown in bold