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. 2015 Aug 20;12:151. doi: 10.1186/s12974-015-0371-z

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© Liu et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — NR1 phosphorylation was increased in the dorsal horn of the spinal cord during colitis. Up-regulation of p-NR1 in the spinal cord during colitis was examined by immunohistochemistry (a–d). The p-NR1 immunoreactivity was increased in the region of the spinal dorsal horn in L1 (a–b) and S1 (c–d) spinal cord during colitis (compare b to a and d to c, circled area). Histograms (e, f) summarized the changes in the relative density of p-NR1 immunoreactivity in the L1 (e) and S1 (f) spinal dorsal horn at 3 days and 7 days of colitis. Microphotographs were from 3 days of colitis and control animals. *p < 0.05 vs. control. Bar = 250 μm