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. 2015 Aug 20;12:151. doi: 10.1186/s12974-015-0371-z

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© Liu et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — BDNF induced NR1 phosphorylation in the spinal cord which was reduced by K252a. Western blot (a) showed that incubation of the acutely cultured spinal cord slices with BDNF (50 ng/mL) elicited an increase in the phosphorylation level of NR1 at Ser⁸⁹⁶ (b). Pre-treatment of the spinal cord slices with a TrkB inhibitor K252a reduced BDNF-elicited NR1 phosphorylation (c). *p < 0.05 vs. control or DMSO alone; #p < 0.05 vs. BDNF (+DMSO)