Table 3. Characteristics of 93 polymorphic SSR markers developed for Carthamus tinctorius L.
| Locus | Repeat motif | Forward primer sequence | Reverse primer sequence | Product size (bp) | Ta (°C) | Allele number (Na) | Gene diversity (He) | Heterozygosity (Ho) | PIC | GenBank accession number |
|---|---|---|---|---|---|---|---|---|---|---|
| NGSaf_2 | (TC)8 | ATCCTACGTGTCAACTTCCCAT | AATCAACAGCAACTAACGCTGA | 251 | 62 | 2 | 0.2580 | 0.0434 | 0.2247 | KM670561 |
| NGSaf_9 | (ACA)7 | AGTCCAACATAGCTCGGATTTC | GACCACTCTCTTCTCCGTCATC | 248 | 62 | 2 | 0.4848 | 0.0434 | 0.3673 | KM670568 |
| NGSaf_12 | (CGG)7 | TTCATCAACAAGTTACAAGGGC | ATTCCCTCTCCTACCCACCTAC | 288 | 62 | 2 | 0.3402 | 0 | 0.2823 | KM670571 |
| NGSaf_13 | (AGC)7 | GGCACAAAGGGATGTTTCTT | TTCTATGATGGGAGGAACTGGT | 108 | 58 | 2 | 0.0831 | 0 | 0.0797 | KM670572 |
| NGSaf_14 | (CAT)7 | ACCCATGTCAATGTACTCTTCGT | AATCTCCTCCATCTCCTCATCA | 196 | 58 | 2 | 0.1219 | 0.0434 | 0.1144 | KM670573 |
| NGSaf_15 | (TGT)7 | CACTCCCTCGTGCAGCTT | ATTCCCAATGCTCCAACAATC | 141 | 60 | 2 | 0.3147 | 0.0434 | 0.2652 | KM670574 |
| NGSaf_20 | (CATT)5 | AGCAAAGTGCTTGCTGACAA | AAGTTCAATTGAGCCCGATG | 243 | 60 | 2 | 0.4990 | 0 | 0.3745 | KM670579 |
| NGSaf_22 | (AG)9 | CCCTCGAGTAAAACTCAAGTCA | AAATGGATTGGTGGGTTTCA | 151 | 60 | 3 | 0.2344 | 0 | 0.2200 | KM670581 |
| NGSaf_23 | (AC)9 | ATATCCTCCTCCGCCGATAA | ATCAACAGGCGTCTCAACCT | 169 | 60 | 2 | 0.3856 | 0 | 0.3112 | KM670582 |
| NGSaf_28 | (ATC)7 | TATGATCTTGTCCGGCCTCT | ATGGCGGATGTTCATAAAGG | 177 | 62 | 2 | 0.0831 | 0 | 0.0797 | KM670587 |
| NGSaf_34 | (CAG)7 | CATCAAAATCATTGGTTGCTTG | TCTTTCACACACTTCTAAGGCAAA | 165 | 60 | 3 | 0.5950 | 0 | 0.5261 | KM670593 |
| NGSaf_39 | (GATG)5 | AATGCTCCAGCTTTCGACAT | TTCGGCCTTCGCTATGTAGT | 247 | 60 | 3 | 0.3553 | 0 | 0.3159 | KM670598 |
| NGSaf_43 | (CT)9 | AAAGCGGTGTTAGTGCTGTGTA | TTTATATGGAAGTGGAAAGGGG | 205 | 60 | 2 | 0.0425 | 0.0434 | 0.0416 | KM670602 |
| NGSaf_44 | (AGG)7 | CACTGTTGTGACCCTTGG | CACACACTTCTAAGGCAAACT | 110 | 62 | 2 | 0.4653 | 0 | 0.3570 | KM670603 |
| NGSaf_45 | (GCT)6 | ACGCCTCTTCTTCTTTCCTTCT | CATTCATGGGTTTAGGTGGC | 317 | 62 | 2 | 0.2268 | 0 | 0.2011 | KM670604 |
| NGSaf_48 | (CTT)6 | ACCCTGGATGACTGAAAACCTA | ATCAACACTCAGGCCCATATTC | 259 | 58 | 3 | 0.4990 | 0 | 0.4337 | KM670607 |
| NGSaf_49 | (CAT)6 | CTCCTTTTGCTTTCTTGATTGG | TCACTCTTGCTGATTTGCTTTG | 231 | 60 | 3 | 0.5368 | 0 | 0.4322 | KM670608 |
| NGSaf_56 | (GAT)6 | GCTAAAACAAGTGTGGCATCAA | AAGATGAAACCTCCCTCAAATG | 172 | 60 | 2 | 0.1587 | 0 | 0.1461 | KM670615 |
| NGSaf_63 | (GAA)6 | ATCCGTTCTTCTTCTAGCACCA | CAGGTGGCATGTATTTTGTTTG | 356 | 60 | 2 | 0.3147 | 0.0434 | 0.2652 | KM670622 |
| NGSaf_65 | (CTC)6 | GAGTGAAAAGAGGGAAGGGTTT | GGGGTTTGGAAAGGGTATTTA | 144 | 60 | 2 | 0.2580 | 0.0434 | 0.2247 | KM670624 |
| NGSaf_67 | (AGG)6 | CTGTTCCACAAGACAAAAGCAA | TCAAGTCCCAATCTCAACCTTC | 277 | 58 | 2 | 0.3147 | 0.0434 | 0.2652 | KM670626 |
| NGSaf_69 | (TAC)6 | TCTCATCAACGATAAAGCAGAATC | TCAACTTCATCTTTTCACGATTTC | 299 | 62 | 3 | 0.6353 | 0.0454 | 0.5602 | KM670628 |
| NGSaf_73 | (GTT)6 | CCAAGGTGACGTGTTGTTCTT | CATCCTTCGATCCACGATAACT | 225 | 60 | 2 | 0.1587 | 0.0869 | 0.1461 | KM670632 |
| NGSaf_83 | (AGA)6 | GCCAAACCCTAACACAGAATCA | CGGTTGTGCCCTAGCTTTTA | 400 | 62 | 2 | 0.4914 | 0 | 0.3707 | KM670642 |
| NGSaf_84 | (TCA)6 | CGCCATCTCTCTCCTCTTCTTA | GGTGTGGAATGGAATGATGATA | 133 | 60 | 2 | 0.3147 | 0.1304 | 0.2652 | KM670643 |
| NGSaf_89 | (TCT)6 | CTCGTAGCTGAGTTTATCGGTG | TGATTGCAGAGAGACTTGTTGA | 169 | 60 | 2 | 0.4536 | 0 | 0.3507 | KM670648 |
| NGSaf_91 | (TAT)6 | CCACTTCTAGTTCGGGTTTCTG | CTGCTGTCATTTCATAGGGTTG | 346 | 58 | 2 | 0.0425 | 0.0434 | 0.0416 | KM670650 |
| NGSaf_92 | (TAC)6 | AGAGGAGTCGATCTTGTGAAGG | GAGAGGTGATACGAGAAGCCAT | 107 | 58 | 2 | 0.2268 | 0 | 0.2011 | KM670651 |
| NGSaf_94 | (TCC)6 | CCATCGAAACTCTACAAAACCC | AGGACAAAAGAGGGAATGATGA | 400 | 60 | 2 | 0.4054 | 0.0434 | 0.3232 | KM670653 |
| NGSaf_98 | (TAC)6 | ATTCTCTGCATGTGGCTTTTCT | CTTGGTTACGGAGGAAGATTTG | 276 | 60 | 2 | 0.4834 | 0 | 0.3665 | KM670657 |
| NGSaf_101 | (TAT)6 | GATTCCGTGCATTCTACACAAA | GAGGAACGAACTAGGAAGGGTT | 141 | 62 | 3 | 0.4158 | 0 | 0.3741 | KM670660 |
| NGSaf_105 | (TGG)6 | CTGTAATTCTGCAACTGAGACCC | GAAGCCATTTTCCACGATTTC | 157 | 60 | 2 | 0.3856 | 0 | 0.3112 | KM670664 |
| NGSaf_111 | (CAA)6 | AATACCCCGCCATCATAGATTA | GAGCTATTCGACAACCAAATCC | 201 | 62 | 2 | 0.4914 | 0 | 0.3707 | KM670670 |
| NGSaf_114 | (AAT)6 | AGACAAATGCAACCACATTCAC | TGTTTATGATCCTTTCAGCCG | 377 | 60 | 2 | 0.1587 | 0 | 0.1461 | KM670673 |
| NGSaf_115 | (CTG)6 | CCATCCAATACTGCAAATCTCC | AGTACCAGCAAGCTCCACCTC | 240 | 62 | 2 | 0.0425 | 0.0434 | 0.0416 | KM670674 |
| NGSaf_117 | (GAA)6 | TTCCATTGAGTCCCATGAAGAT | TCTCTGTTCCACGTAGGGCT | 190 | 62 | 2 | 0.0831 | 0 | 0.0797 | KM670676 |
| NGSaf_130 | (TGG)6 | TGCGACTTGTGTTTCTTCTTCCC | AAAAGCCGTCCGGTGAAATTG | 371 | 62 | 2 | 0.4234 | 0 | 0.3337 | KM670689 |
| NGSaf_138 | (TGT)6 | AACCTGTGTACCATCTGCTAATTG | ATGAGATCCGAAGTCCATTGTT | 160 | 60 | 2 | 0.0831 | 0 | 0.0797 | KM670697 |
| NGSaf_142 | (TGT)6 | GATGTTAACCTGTGTACCATCTGC | CGTCTAATGAACACTCAATCCAAA | 442 | 60 | 3 | 0.4473 | 0.0454 | 0.3655 | KM670697 |
| NGSaf_145 | (GGA)6 | GAGCATGAAACGGAGAATTAGG | TCAACAGTAGCAGATCCTTCCA | 358 | 60 | 2 | 0.4990 | 0 | 0.3745 | KM670703 |
| NGSaf_148 | (CAT)6 | CCATGATTTTATGCTCATCGTG | AGCAATAGCAGGTGCAGTGATAG | 366 | 62 | 2 | 0.4536 | 0 | 0.3507 | KM670706 |
| NGSaf_151 | (GAT)6 | AGTCTTGGCCTGTAACCACTTATC | CATGAAGAGACGTTTGAAGCAG | 377 | 60 | 2 | 0.4914 | 0 | 0.3707 | KM670709 |
| NGSaf_152 | (CAA)6 | GTTGTTCGTTTGCCCCAATCTG | CAGAGCCACAAGATGCCGAATTA | 100 | 62 | 3 | 0.4682 | 0.0952 | 0.4149 | KM670710 |
| NGSaf_154 | (GAT)6 | TGATATCAATGGTATGATTTTCCTTT | GGATGGCGAACAAGATTACAA | 500 | 62 | 4 | 0.5826 | 0 | 0.4938 | KM670712 |
| NGSaf_155 | (AAG)6 | AAGTATTTGAACAAGTGTACCGGC | AGATGATGAAGGAAGGAGGTAATG | 335 | 58 | 2 | 0.3367 | 0 | 0.2800 | KM670713 |
| NGSaf_156 | (CAC)6 | AACTGCTTCTAGGGTTTTCCTCTT | CCCCAAATTACCAACTTCCATAC | 294 | 62 | 3 | 0.2637 | 0.0434 | 0.2385 | KM670714 |
| NGSaf_158 | (TAA)6 | ATGTTTGTCCCACTCGGTCTTC | TAAGTAGCTGAAGTGCAAGGTCGT | 274 | 60 | 2 | 0.1219 | 0.0434 | 0.1144 | KM670716 |
| NGSaf_164 | (TGT)6 | CATCTCAGCCTTTCTGTCTCCT | AAAATCGGTTGTTTGGTTGC | 313 | 60 | 2 | 0.2268 | 0 | 0.2011 | KM670722 |
| NGSaf_173 | (AGC)6 | GTGGTTCGAGTCTGTTTATTTCCA | CTGCACTTTTGAGTTGTGTGTGAT | 351 | 60 | 2 | 0.4536 | 0 | 0.3507 | KM670731 |
| NGSaf_178 | (ATC)6 | TAAGAAAGGGCACCATTGAAGTAG | GATATGAGCAGAGGAGTTTGTGC | 128 | 62 | 2 | 0.4763 | 0 | 0.3629 | KM670736 |
| NGSaf_181 | (GTA)6 | TATGGTGATCGAAGAAGAAGACAG | ACTGAGCAATGAAGAGTTCCAGA | 299 | 58 | 3 | 0.3279 | 0.0434 | 0.2954 | KM670739 |
| NGSaf_201 | (TAT)6 | GTTATTGTTGTCCGTGCAAGTAGT | GCTTGGTTCCTAGTCGTAGTTCAT | 309 | 58 | 2 | 0.4914 | 0.0869 | 0.3707 | KM670739 |
| NGSaf_204 | (AAC)6 | GCCATGCCCATATACAAACAGATA | AAGAAATGGTTCCACCGAGTCA | 350 | 60 | 2 | 0.3856 | 0 | 0.3112 | KM670762 |
| NGSaf_210 | (TCT)6 | TGATAGTAGCTTATTCCCTCAGCC | AACGGTGGTAGGATAGTTGACG | 199 | 60 | 2 | 0.4977 | 0 | 0.3738 | KM670768 |
| NGSaf_211 | (GAT)6 | GGTCTGCAAGAGTAAGTGGGAG | CCAAATCCCTGCTACAAAACAT | 132 | 60 | 2 | 0.4958 | 0.0909 | 0.3729 | KM670769 |
| NGSaf_236 | (ATC)6 | ACCTTGAGGGGTATTTGGGTAA | GTTGGTAGGGTGATCTTCGGT | 293 | 62 | 2 | 0.4921 | 0 | 0.3710 | KM670794 |
| NGSaf_237 | (ATT)6 | GACATTCCACTTATGCCGGTAG | TGGGACAATGACTCTGTTTGAG | 250 | 62 | 2 | 0.4962 | 0.9130 | 0.3731 | KM670795 |
| NGSaf_238 | (CAT)6 | AACAGTGGGCCTGATATGTTTT | CGGCTAATCCAAACCCTAGAAT | 343 | 62 | 2 | 0.4395 | 0.0434 | 0.3429 | KM670796 |
| NGSaf_239 | (ATT)6 | CAAAACTTCTCAACCGTGAAC | GTAACATACCGATTTTCTTGGC | 133 | 62 | 2 | 0.2777 | 0.2380 | 0.2391 | KM670797 |
| NGSaf_242 | (AAG)6 | GGAGGAGAGATTGAAGGTGAAG | CCAACCATCGTCTGAACTTTT | 129 | 62 | 2 | 0.1937 | 0.0434 | 0.1749 | KM670800 |
| NGSaf_245 | (ATG)6 | TGAACATGGTAAAAACCCATACA | TCTCAAAGGAATGGGAATGG | 109 | 62 | 2 | 0.4938 | 0 | 0.3718 | KM670803 |
| NGSaf_248 | (TCT)6 | CTGCATTTCTTCCCCATTATTATC | GTGTTTAAGGTTTCATTGCCTTCT | 173 | 62 | 2 | 0.0831 | 0 | 0.0797 | KM670806 |
| NGSaf_255 | (TCA)6 | AGGTGTTGGGAGGACACTAAAA | CCGCTCTTAGCTAAACTCTTGC | 224 | 62 | 2 | 0.4848 | 0.0434 | 0.3673 | KM670813 |
| NGSaf_257 | (ACA)6 | ACAGCACCTGCCATCTTCATTA | AGGCATGAGCTTCGATTTTAGC | 239 | 62 | 2 | 0.3856 | 0 | 0.3112 | KM670815 |
| NGSaf_259 | (ATT)6 | ATCGGGCATCCACTACTAGCTT | TGTGAAGTGAAAGATGAAATTCAAGA | 236 | 62 | 2 | 0.4763 | 0 | 0.3629 | KM670817 |
| NGSaf_261 | (TTC)6 | CGAATCTACCAAGATCTACCAAATCC | GATATGGCGGAGAAACCGTAAA | 102 | 60 | 2 | 0.4990 | 0.0869 | 0.3745 | KM670819 |
| NGSaf_262 | (TAT)6 | AAACCTGTACGGACACGTATCAA | CGAATTCCATCTCGATTTCTATATTTG | 132 | 60 | 3 | 0.5207 | 0.0434 | 0.4059 | KM670820 |
| NGSaf_264 | (TTA)6 | GATGATGAATTTTGGTCGAACG | CGTTTAATTGACGATAATGCATGTG | 152 | 62 | 3 | 0.5051 | 0.0454 | 0.4511 | KM670822 |
| NGSaf_265 | (AAT)6 | GGTAAAGACGAAGTTCACGATGG | TCTATCGCCGCCTTCTTCAC | 155 | 62 | 2 | 0.2508 | 0.0588 | 0.2193 | KM670823 |
| NGSaf_266 | (TGA)6 | TGGGAGTAAATTGGTGTTGAAGC | AAACGAAAACGGGAGAGATGAA | 211 | 62 | 3 | 0.1975 | 0.0434 | 0.1847 | KM670824 |
| NGSaf_273 | (GAA)6 | ATAGTAATTTGTAGCGAAAAGCGAAA | TGGATTCCAGGATTAACATATTCTT | 150 | 62 | 2 | 0.4914 | 0 | 0.3707 | KM670831 |
| NGSaf_276 | (AGA)6 | CCCATTTCAACTAAATTTCAAGC | TCTTCTTTCTGGGTGGCTTC | 110 | 62 | 2 | 0.0867 | 0 | 0.0830 | KM670834 |
| NGSaf_279 | (AAG)6 | AGCCTCGAGTTAAGCCATGA | TTCACGCGTTCTCTGTAGGA | 154 | 60 | 2 | 0.3254 | 0.0454 | 0.2724 | KM670837 |
| NGSaf_281 | (AG)22 | CAGGGACATCAGTTTGAGGAG | TGTTGGTGCTAACAAAGAACC | 108 | 62 | 3 | 0.6258 | 0 | 0.5509 | KM670839 |
| NGSaf_282 | (AG)18 | TACCCTCAATGGGAGTACCG | GGTCATGAGCTCGAATGAGG | 185 | 58 | 3 | 0.405 | 0 | 0.3679 | KM670840 |
| NGSaf_286 | (GA)16 | GCAAAATGGGAACACATTCA | TCTCCAGTGTAGCGTGTGAAA | 243 | 58 | 3 | 0.6124 | 0 | 0.5432 | KM670844 |
| NGSaf_289 | (AT)16 | TGCCATTTCATTAGTCACGA | ACTTGTTTTTACATCGAATCCTTT | 139 | 60 | 3 | 0.6200 | 0.9565 | 0.5435 | KM670847 |
| NGSaf_292 | (CT)16 | CGTAACCACATAATACTTGGAGAA | TCCATATCCTTGAAAACCCATT | 175 | 62 | 2 | 0.3856 | 0 | 0.3112 | KM670850 |
| NGSaf_294 | (CA)15 | AAGTAACAGATAAGATTTCAACAGCTC | CGATGGTATGAATAGTCGTCGT | 155 | 62 | 3 | 0.3494 | 0.0714 | 0.3084 | KM670852 |
| NGSaf_295 | (CT)15 | GCCTCTGCCTCTCTCTCTATTTT | CCTGTCGGTAGATCGAAGAAG | 131 | 62 | 2 | 0.4716 | 0 | 0.3604 | KM670853 |
| NGSaf_296 | (TC)15 | TCTCTGACGCTATGGGACAA | TGTGAGGATATGTAGAATCCCATT | 158 | 62 | 2 | 0.4536 | 0 | 0.3507 | KM670854 |
| NGSaf_300 | (CA)13 | TGCCTGATTCTTCACCTTCTC | TTAGCTGAAGCACTTTTGCTCTT | 100 | 62 | 3 | 0.5141 | 0 | 0.4608 | KM670858 |
| NGSaf_301 | (CA)12 | TCTTTTCAAGTGTTGCATGAGC | TGATGGTCTTCTCGCTACCA | 249 | 60 | 2 | 0.4567 | 0 | 0.3524 | KM670859 |
| NGSaf_306 | (CT)14 | GTCCAAGACCCGAGATGAAG | AGATTGGATTGCCGATAAATG | 152 | 60 | 2 | 0.1244 | 0 | 0.1167 | KM670864 |
| NGSaf_307 | (CT)14 | ACCCAAATCATGCTTCAACA | GGTGTGGATAATCAACCACTTTC | 151 | 58 | 2 | 0.3046 | 0 | 0.2582 | KM670865 |
| NGSaf_308 | (CT)13 | TAACCCGCATTCTTGGTTTT | CCCTATCTGTGTGAGCCTGA | 157 | 60 | 2 | 0.4990 | 0 | 0.3745 | KM670866 |
| NGSaf_309 | (GA)18 | TTGCAACCCTTCAACAAAAA | TCGAAGCCTTACCTTCCTCA | 172 | 62 | 3 | 0.4390 | 0.0454 | 0.3746 | KM670867 |
| NGSaf_310 | (GA)16 | GTCGATGGGAGTTTGAGAGG | CGATTGTGAGAGGGGTAGGA | 204 | 62 | 2 | 0.4958 | 0 | 0.3729 | KM670868 |
| NGSaf_313 | (GT)13 | GGGGTTGTTTTCCAAGAGTTT | CAGACAACTTTATCCAACATAGGAC | 155 | 60 | 3 | 0.4297 | 0 | 0.3854 | KM670871 |
| NGSaf_314 | (GT)12 | TTTTCCGACTAAAACCCCTTT | AGGGTGTTACACGCAAACTTC | 100 | 62 | 3 | 0.4917 | 0 | 0.4076 | KM670872 |
| NGSaf_322 | (TC)15 | AGCCGAAGAGCAAGCAAAT | GAGATGTTATGGCGGTGGTG | 105 | 62 | 2 | 0.5 | 0 | 0.375 | KM670880 |
| NGSaf_323 | (TC)13 | AAAGACTGTATTTGGGCTACGG | TTGAGATGCAACATGAATTGAA | 168 | 62 | 2 | 0.4501 | 0.6842 | 0.3488 | KM670881 |
| NGSaf_324 | (TG)15 | TGTTTTCCGGTTATTTGAGGTT | GAAGATGCTCTAACGGACCAA | 156 | 60 | 4 | 0.6228 | 0.0434 | 0.5457 | KM670882 |
| Mean | 2.29 | 0.3746 | 0.0494 | 0.3075 |