Fig. 1.

Experimental Design. Adult C57BL/6Kuo mice (20 each male and female) were divided into five treatment groups per sex. Each group received a single dose of TCDD in corn oil (125, 250, 500, or 1000 μg/kg) or corn oil alone. Livers were excised 4 days after treatment and RNA was isolated and hybridized to Affymetrix Mouse Gene 1.1 ST arrays. Data for each sex were pre-processed and modelled separately. Results for male and female cohorts were then combined. Those genes determined to be significantly altered by treatment were identified and downstream analyses, including pattern recognition and function analyses, were performed. A meta-analysis was performed through integration of data from 13 rodent-TCDD studies and additional analyses were performed to identify trends