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. Author manuscript; available in PMC: 2015 Aug 22.
Published in final edited form as: Integr Biol (Camb). 2015 Jul 6;7(7):815–824. doi: 10.1039/c5ib00105f

Fig. 3.

Fig. 3

Hepatocytes atop col/HGF spots (HGF-stimulated) rescue hepatic phenotype in neighboring cells on collagen (recipients) via cell secreted factors. (A) Expression of albumin in primary hepatocytes (PH) cultured on collagen and col/HGF spots inside microfluidic chambers analyzed by qPCR. (B) Immunofluorescence staining reveals high levels of albumin in recipient cells in three different conditions: recipient cells next to HGF-stimulated hepatocytes, col/HGF spots (reservoir), and another group of recipient cells. (C) Albumin expression analysis by qPCR in recipient hepatocytes cultured next to collagen black spots, HGF reservoirs, and HGF-stimulated hepatocytes. (D) Albumin production by recipient hepatocytes cultured next to collagen black spots and HGF reservoirs, showing there not influence on rescuing functionality by HGF released from reservoir. Results are represented as mean±SD (n=3). *: p-value < 0.05. NS: Non-significant.