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. 2015 Aug 21;8:59. doi: 10.1186/s13048-015-0173-z

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© Fu et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Establishment of stable CLU silencing OVCAR-3 and TOV-21G cell lines. a Detection of lentivirus transfection in OVCAR-3 and TOV-21G cells. The results of gene sequencing showed that OVCAR-3 and TOV-21G cells were transfected successfully LV-CLU or LV-NC gene with lentivirus (b) Analysis of levels of Clusterin protein in OVCAR-3 and TOV-21G cells. Results suggested that both OVCAR-3 and TOV-21G cells had the higher level of Clusterin expression. c Clusterin (GFP) expression in OVCAR-3 and TOV-21G cells as detected by fluorescence microscope (x100). The infection efficiency of OVCAR-3 cells (MOI 20) and TOV-21G cells (MOI 10) were shown. The cells were infected with LV-CLU or LV-NC. Four days later, the infection efficiency achieved was >90 %