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. 2015 Aug 22;15:208. doi: 10.1186/s12870-015-0591-5

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© Han et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Expression level of CiNAC3 or CiNAC4 in their overexpression lines. The T3 transgenic plants growing under normal condition was detected by relative quantitative real-time PCR. There was no amplification in Col-0. Expression values were calculated using 2^-ΔCTmethod and AtEF1α as endogenous control. Two independent biological replicates were performed with similar result.Three technical replicates of each biological replicate were analyzed in quantitative real-time PCR analysis