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. 2015 Aug 20;10(8):e0135830. doi: 10.1371/journal.pone.0135830

Fig 4. VSX2-positive hRPCs from short term cultures of human prenatal retinal neurospheres express ASCL1 upon differentiation.

Fig 4

(A) VSX2+/ASCL1+ hRPCs were present in 90 day gestation human prenatal retinal tissue. (B) The percentage of ASCL1+ cells in short term prenatal human retinal neurosphere cultures was determined by immunocytochemistry after mitogen withdrawal. ASCL1 immunoreactivity co-localized with (C) VSX2, (D) KI67, and (E) SOX2, but not with (F) ßIII TUBULIN. (G) The percentages of ASCL1+ cells that co-labeled with individual progenitor markers after differentiation was determined. Nuclei were visualized with DAPI. *p<0.05. Arrows in panels A and C-E designate ASCL1+ nuclei. Scale bar: 50 μm (panel A); 20 μm (panels C-F).