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. 2015 Aug 20;10(8):e0135830. doi: 10.1371/journal.pone.0135830

Fig 6. VSX2+ hRPCs are abundant within short and long term cultures of 3D optic vesicles derived from hESCs.

Fig 6

20 days after initiation of retinal differentiation [20], WA09 hESCs formed optic vesicle structures (OVs) comprised of VSX2+ hRPCs co-expressing (A) KI67, (B) NESTIN, and (C) SOX2. (D-F) At day 50, the majority of VSX2+ hRPCs remained KI67+. (G) VSX2+/KI67+ progenitors were also present at day 50 in hESC-OVs derived from the WA01 line. 50 day VSX2+ hRPCs continued to express (H) NESTIN and (I) SOX2. (J) At 20, 50, and 90 days of differentiation, the percentages of VSX2+ or KI67+ cells and (K) VSX2+ cells co-labeled with other progenitor markers were quantified. Nuclei were visualized with DAPI and cell count data is expressed as % immunopositive cells. Scale bar: 50 μm (panels A-D,H,I); 20 μm (panels E,F,G).