Fig. 6.

GRAF1 partial depletion increases EBD accumulation and myofiber regeneration in young mdx muscle. a Representative images of EBD (red) accumulation in tibialis anterior muscle of 3-week-old littermates with indicated genotypes. The mice were injected with EBD 24 h prior to tissue harvest. Nuclei are counterstained with DAPI (blue). b Three-week-old tibialis anterior muscle immunostained with eMHC (green) to demarcate regenerative fibers. Wheat germ agglutinin (WGA; red) demarcates myofiber boundaries. Nuclei are counterstained with DAPI (blue). c, d Percentage of EBD- and eMHC-stained tibialis anterior myofibers, respectively, (*p < 0.005; N = 3 per genotype). Data are represented as mean ± sem. Scale bars = 100 μm