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. 2015 Aug 21;5:27. doi: 10.1186/s13395-015-0054-6

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© Lenhart et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — GRAF1 associates with dysferlin in injured/dystrophic muscles and promotes its recruitment to the PM. a Immunohistochemical analysis of GRAF1 expression (green) in normal and dystrophic (golden retriever muscular dystrophy; GRMD) muscle. Co-staining with dysferlin (red, left) and α-actinin (red, right) demonstrates localization of GRAF1 in striated pattern aligned with Z-disks in normal muscle compared to localization to the plasmalemma in GRMD muscle (note intramuscular inflammatory infiltrates indicative of muscle injury as visualized by DAPI staining). b Anti-GRAF1 rabbit polyclonal antibody [52] immunoprecipitation (IP) from adult quadriceps muscle 7 days following CTX-induced injury. Blots were probed with an anti-dysferlin antibody or hamster anti-GRAF1 antibody. Input contains 2 % of cellular lysate used for IP. c Immunohistochemical analysis of dysferlin (red) in Graf1^Wt/Wt and Graf1^Gt/Gt adult mouse hearts indicates dysferlin mis-localization in the absence of GRAF1. Nuclei are counterstained with DAPI (blue). d Immunohistochemical analysis of dysferlin (red) and GRAF1 (green) reveals plasmalemmal and sarcomeric localization respectively in Wt hearts. Scale bars = 20 μm