Table 4.

Timeline showing timings of blood and nasal sample collection from calves

Intranasal treatments (6 animals/group)
Commercial Vaccine	Empty NPs	BPI3V proteins	PLGA-BPI3V-NPs
Blood collection	Nasal mucus collection
Day 0 (Immunisation)	Day 0 (Immunisation)
Day 7 (1 week PI)	Day 7 (1 week PI)
Day 14 (2 weeks PI)	Day 14 (2 weeks PI)
Day 21 (3 weeks PI)	Day 21 (3 weeks PI)
Day 28 (4 weeks PI)	Day 28 (4 weeks PI)
Day 35 (Booster inoculation)	Day 35 (Booster inoculation)
Day 42 (1 week PB)	Day 42 (1 week PB)
Day 49 (2 weeks PB)	Day 49 (2 weeks PB)
Day 56 (3 weeks PB)	Day 56 (3 weeks PB)
Day 63 (4 weeks PB)	Day 63 (4 weeks PB)
Day 71 (n = 3, euthanised)	Day 71 (n = 3, euthanised)
Day 76 (5 days PE)	Day 76 (5 days PE)
Day 83 (12 days PE)
Day 85 (2 weeks PE)	Day 85 (2 weeks PE)
Day 92 (3 weeks PE)	Day 92 (3 weeks PE)

PI Post immunisation, PB Post booster, PE Post euthanasia