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. 2015 Aug 20;10(8):e0136292. doi: 10.1371/journal.pone.0136292

Fig 6. Activity of CTLs from peripheral blood and GALT of HIV controllers and progressors.

Fig 6

(A-D) Peripheral blood and rectal cells were stimulated with HIV-1 Consensus B Gag-peptide pool during 12 h, and then monoclonal antibodies against TNFα, MIP-1β, IL-2, CD107a, IFN-γ, granzyme B and perforin, were added. The expression of these molecules was detected by flow cytometry as described in Materials and Methods. The results are presented as median, range minimum and maximum. (E) Comparison of the polyfunctional profiles of Gag-specific responses in PB. The slices of the pies correspond to the proportions of Gag-specific CD8+ T-cells (as a frequency of the total Gag response) expressing 1, 2, 3, 4, or 5 functions, calculated using Boolean gating. A Mann Whitney test was used with a confidence level of 95%. Significant differences are indicated at the top of the figure. (*p<0.05, **p<0.01).