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. 2015 Aug 20;10(8):e0136109. doi: 10.1371/journal.pone.0136109

Fig 1. High-throughput expression and detection of P. falciparum proteins.

Fig 1

Compartmental wheat germ expression of recombinant proteins represented by five clones as GST (A) and 6xHis fusions (B). [C14]-Leucine labeled proteins detected by radio blot in three fractions; total (T), supernatant (S), and pellet (P). Protein predicted molecular weight sizes are: (1) 32.8 kDa, (2) 50.2 kDa, (3) 37.7 kDa, (4) 59.7 kDa and (5) 32.5 kDa. The molecular sizes for GST-fused proteins (A) include an additional 29 kDa (GST molecular size). The autoradiographs for all the 151 clones are shown in S1 Fig.