Fig 3. Stage-specific expression of CSP and CelTOS by immunofluorescence IFA and immuno-electron microscopy.

CSP and CelTOS were localized to sporozoites (A, B, D, and E) and 7-day old liver stages (C and F) by IFA (A, C, D, F) and by immune-electron microscopy (B, E). CSP: localized to the sporozoite surface, (arrows, A), outer sporozoites membranes and shed material (arrows B), and the periphery of 5 day liver stage (arrows, C). CelTOS: localized to patches within sporozoites (arrows, D) that are associated with micronemes (arrows, E), was not on the surface, and was in the cytoplasm of five day liver stages (arrow, F).