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. 2015 Aug 20;10(8):e0136109. doi: 10.1371/journal.pone.0136109

Fig 5. Stage-specific expression of three novel P. falciparum antigens by immunofluorescence and immune-electron microscopy.

Fig 5

Three additional novel P. falciparum antigens (Pf106, Pf121, Pf144) were localized by immunofluorescence to sporozoites (M, Q, U), 7-day liver stages (O, S, W), and blood stages (P, T, X); and by immuno-electron microscopy to sporozoites (N, R, V). Pf106: localized in clumps at the sporozoite anterior pole (arrow, M), in vesicles inside the sporozoite (arrows, N), in liver stage merozoites (arrows, 0), and in blood stage merozoites (arrows, P). Pf121: localized unevenly in the sporozoite periphery/interior (arrows, Q) that appear to localize to micronemes (arrows, R), throughout liver stage parasite (arrow, S) and in clumps in blood stage schizonts but not the residual body (arrows, T). Pf144: localized to clumps inside the sporozoite (arrow, U) that are associated with micronemes (arrows, V), but is only weakly detected in 7-day liver stage parasites (arrow, W) and is dispersed in the blood stage schizonts (arrow, X).