Table 1. Primer sequences, amplicon and intron lengths, squared correlation coefficient and qPCR efficiency.
| Gene | Primer sequence (5'to 3') | Amplicon length (bp) | Intron length (bp) | R2 | Eand |
|---|---|---|---|---|---|
| Sequencing primers | |||||
| for-fragment 1 | For- AGGTGTTCGGAGAGTTGGCGATA | 1100 | - | - | - |
| Rev- GCTACATACTCCGGCGTACCAC | |||||
| for-fragment 2 | For- CCTGTTACTGGACGTTAGCGGTTA | 429 | - | - | - |
| Rev- AAATCCGCATCCCAACCAGTGA | |||||
| qPCR primers | |||||
| foraging | For- AGTTCGAAGGTCTGCGACTG | 191 | 3064 | 0.99 | 1.1 |
| Rev- GCCCATGATCTCCTTCTCGG | |||||
| β-Actin | For- TGTCTCCCACACTGTACCCATCTA | 87 | 338 | 1 | 0.97 |
| Rev- TCGGTAAGATCACGACCAGCCAA | |||||
| GAPDH | For- GACTGGCATGGCATTCAGAGTT | 182 | 1130 | 0.99 | 0.91 |
| Rev- CCCCATTAAAGTCCGATGACACC | |||||