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. 2015 Aug 20;10(8):e0136167. doi: 10.1371/journal.pone.0136167

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 3 — Rats were euthanized 30 min after one injection of methamphetamine (2 mg/kg, i.p.) or vehicle, and striatal homogenates were fractionated by differential centrifugation. S3 = cytosol, P1 = nuclei and large debris, P2 = crude synaptosomes, LP1 = synaptosome membranes, LP2 = synaptic vesicles, P3 = light membranes (non-synaptosome). The fractions and initial homogenates, containing equal amounts of protein, were loaded into SDS-PAGE gels and analyzed by western blot for Rab10 immunoreactivity. Results shown are the mean ± SEM of 4 samples, expressed as the ratio of Rab10 immunoreactivity in each fraction over Rab10 immunoreactivity in the initial homogenate. *p < 0.05 (unpaired Student’s t-test).