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. 2015 Aug 20;10(8):e0136167. doi: 10.1371/journal.pone.0136167

Fig 5. DN-Rab10 inhibits methamphetamine-induced mortality.

Fig 5

Methamphetamine was added to the food at 3 or 6 mg/ml and the number of flies surviving over the next 8 days was determined. Death was recorded at the time activity was no longer detected. (A) The median survival time was 2.8 days for control (Appl-GAL4) flies fed 6 mg/ml methamphetamine and 4.1 days for flies expressing DN-Rab10 in all neurons (Appl>DN-Rab10). p = 0.0012 for comparison of the curves (Mantel-Cox log-rank test). The typical % survival over the same time period in flies not fed methamphetamine is greater than 90% (not shown). (B) The median survival time was 2.9 days for control flies (UAS-DN-Rab10), 6.5 days for flies expressing DN-Rab10 in all neurons (Appl>DN-Rab10) and 3.6 days for flies expressing DN-Rab10 in cholinergic neurons (Cha>DN-Rab10) and fed 3 mg/ml methamphetamine. P < 0.0001 for the comparison of the control and Appl>DN-Rab10 curves and p = 0.49 for the comparison of the control and Cha>DN-Rab10 curves.