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. 2015 Aug 20;10(8):e0136442. doi: 10.1371/journal.pone.0136442

Fig 2. Effects of Gb3 and lyso-Gb3 treatments on EMT through PI3K/AKT pathway in HK2 cells.

Fig 2

(A–C) Immunoblotting analysis of TGF-β, EMT markers, and phosphorylation of PI3K/AKT following Gb3 (30 μM and 45 μM) treatment of HK2 cells for 72 h. (D–F) Immunoblotting analysis of TGF-β, EMT markers, and phosphorylation of PI3K/AKT following lyso-Gb3 (200 nM and 400 nM) treatment of HK2 cells for 72 h. Panels (A–F) show a representative experiment (right panel), and data are presented as mean ± SEM, n = 3. Gb3 or lyso-Gb3 vs control, One-way ANOVA. * P < 0.05, ** P < 0.001, and *** P < 0.0001.