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. 2015 Aug 20;10(8):e0136442. doi: 10.1371/journal.pone.0136442

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© 2015 Jeon et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — (A, B) Immunoblotting analysis of TGF-β and EMT markers following Gb3 (30 μM and 45 μM) treatment of SV40 MES 13 cells for 72 h. (C, D) Immunoblotting analysis of TGF-β and EMT markers following lyso-Gb3 (200 nM and 400 nM) treatment of SV40 MES 13 cells for 72 h. (E) The activity of TGF-β and EMT markers was assayed by the immunoblotting method treating of SCDase (250 μU/ml) and Gb3 (30 μM and 45 μM, respectively) for 72 h in SV40 MES 13 cells. Panels (A–E) show a representative experiment (right panel) and data as mean ± SEM, n = 3. (A–D) Gb3 or lyso-Gb3 vs control, (E) Gb3 vs Gb3 + SCDase, One-way ANOVA. * P < 0.05, ** P < 0.001, and *** P < 0.0001.