Table 2.
Antibody | Species, dilution, incubation | Antigen retrieval |
---|---|---|
IRS1 | Rabbit IgG, 1/25, 1 h RT | TSC, pH 6.5, MW 10 min |
pIRS1 S616 | Rabbit IgG, 1/100, 1 h RT | TSC, pH 6.5, MW 10 min |
pIRS1 S636/639 | Rabbit IgG, 1/50, 1 h RT | TSC, pH 6.5, MW 10 min |
pIRS1 Y612 | Rabbit IgG, 1/100, 1 h RT | EDTA, pH 8.0, MW 10 min |
GFAP | Rabbit IgG, 1/500, ON, 4 °C | Dependent on initial probe (above) |
TSC tris-sodium citrate, EDTA ethylenediaminetetraacetic acid, RT room temperature, ON overnight, MW microwave