Table 2.

Immunohistochemistry antibody source and specificity

Antibody	Species, dilution, incubation	Antigen retrieval
IRS1	Rabbit IgG, 1/25, 1 h RT	TSC, pH 6.5, MW 10 min
pIRS1 S616	Rabbit IgG, 1/100, 1 h RT	TSC, pH 6.5, MW 10 min
pIRS1 S636/639	Rabbit IgG, 1/50, 1 h RT	TSC, pH 6.5, MW 10 min
pIRS1 Y612	Rabbit IgG, 1/100, 1 h RT	EDTA, pH 8.0, MW 10 min
GFAP	Rabbit IgG, 1/500, ON, 4 °C	Dependent on initial probe (above)

TSC tris-sodium citrate, EDTA ethylenediaminetetraacetic acid, RT room temperature, ON overnight, MW microwave