Fig 4. Post-fixation Bd detection success (top) and zoospore equivalents (bottom) across all swab events (n = 58).

(a) Bd detection success was significantly greater for swab event E as compared to all other swab events (p < 0.0001). (b) The two ZE outliers in Event E are from specimens that had pre-preservation Bd loads of less than 1 zoospore equivalent and were not detected, in any number of runs, by any of the PM-extracted swabs. Results are based on analysis of singlicate data.