Table 1. Structure of the antigens used in this study.
| Antigen | N-terminus Fusion tag | Coding Region | C-terminus Fusion tag | Accession | MW (kDa)* | |
|---|---|---|---|---|---|---|
| S. mansoni | Cathepsin B (Sm31) | MASWSHPQFEKGALEVLFQGPGYQDP | 1–340 | VDAAAELALVPRGSSAHHHHHHHHHH | AAA29865 | 44.4 |
| Filamin | MASWSHPQFEKGALEVLFQGPGYP | 1,510–2,314 | ELALVPRGSSAHHHHHHHHHH | CCD59036 | 92.1 | |
| GAPDH | MASWSHPQFEKGALEVLFQGPGYQ | 1–338 | ELALVPRGSSAHHHHHHHHHH | P20287 | 41.5 | |
| GST (Sm28) | MASWSHPQFEKGALEVLFQGPGYQDP | 1–211 | AAAELALVPRGSSAHHHHHHHHHH | P09792 | 29.4 | |
| LAP-1 | MASWSHPQFEKGALEVLFQGPGYQ | 1–523 | ELALVPRGSSAHHHHHHHHHH | ACQ77148 | 61.9 | |
| LAP-2 | MASWSHPQFEKGALEVLFQGPGYQ | 1–544 | ELALVPRGSSAHHHHHHHHHH | ACQ77149 | 64.9 | |
| Legumain (Sm32) | MASWSHPQFEKGALEVLFQGPGYQDP | 1–429 | VDAAAELALVPRGSSAHHHHHHHHHH | P09841 | 54.8 | |
| RP26 (Sm22.3) | MASWSHPQFEKGALEVLFQGPGYQ | 20–196 | ELALVPRGSSAHHHHHHHHHH | AAB81008 | 25.3 | |
| SERPIN | MASWSHPQFEKGALEVLFQGPGYQDP | 1–406 | VDAAAELALVPRGSSAHHHHHHHHHH | CCD60071 | 51.8 | |
| Tropomyosin-2 | MASWSHPQFEKGALEVLFQGPGYQDP | 1–284 | VDAAAELALVPRGSSAHHHHHHHHHH | P42638 | 38.5 | |
| S. haematobium | SERPIN | MASWSHPQFEKGALEVLFQGPGYQDP | 1–406 | VDAAAELALVPRGSSAHHHHHHHHHH | AAA19730 | 51.6 |
The cDNAs containing the coding regions of the antigens were cloned into pET52b expression vector with StrepTagII fusion tag at N-terminus and polyhistidine tag at C-terminus.
*Predicted molecular weight