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. 2015 Aug 20;10(8):e0132746. doi: 10.1371/journal.pone.0132746

Fig 3. Inhibition of HIFαusing siRNA suppresses expression levels of miR27a and reduces drug resistance of OCUM-2MD3/L-OHP cells.

Fig 3

OCUM-2MD3/L-OHP cells were transfected with either HIF1α-siRNA or NS-siRNA (A), or transfected with 20, 40 or 80 nM of HIF1α-siRNA-2 (B), or transfected with 80 nM HIF1α-siRNA-2 for 24, 48 or 72 h (C), then cells were collected for Western blotting assays to calculate the knockdown efficiency. miR-27a levels were determined by qPCR (D). The survival rate of OCUM-2MD3/L-OHP was estimated by MTT assay.(E)Results were presented as the mean ± S.D. (n = 3). *P<0.01 compared with NS-siRNA group.