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. 2015 Aug 20;11(8):e1005453. doi: 10.1371/journal.pgen.1005453

Fig 6. sim-bam-yfp; bam −female fertility decreases with additional copies of sim-bam-yfp.

Fig 6

(A, B) Fertility comparison of bam −flies with one versus two copies of mel-bam-yfp (A) or sim-bam-yfp (B). For A and B, one female and two tester males were allowed to mate and the trio was transferred to a new vial every five days. Fertility is shown as the average number of progeny per female +/- SEM for each vial. (A) N ranged between 38 and 40 females at start of experiment; due to female mortality N ranged between 26 and 32 at end of experiment. (B) N ranged between 36 and 40 females at start of experiment; due to female mortality N ranged between 32 and 33 at end of experiment. All mel-bam-yfp; bam −comparisons are not significant while all sim-bam-yfp; bam −comparisons between one and two copies are highly significant (t-test, ***P<0.001). (C) An ovariole from sim-bam-yfp; bam −that has only a single GSC (arrow). (D) An ovariole from 2x sim-bam-yfp; bam −showing a complete loss of GSCs and germline as indicated by lack of Vasa staining. Asterisk indicates anterior end of germarium where GSCs normally reside. (E) A 2x sim-bam-yfp; bam/+ ovariole shows a more wildtype ovary morphology compared to its 2x sim-bam-yfp; bam −sibling. For C-E, ovaries are from flies aged for 3–5 days post-eclosion and are stained for Vasa (green), Hts-1B1 (red), and YFP (blue). Scale bar, 50μm.