Fig 7. sim-Bam maintains interactions with mel-Bgcn in immunoprecipitates from S2 cells.

(A-B) Control experiments with mel-Bam and mel-Bgcn. (A) Cells were transfected with mel-Bam::HA and either mel-Bgcn::MYC or MYC. Anti-MYC immunoprecipitates were analyzed by Western blot. (B) Cells were transfected with mel-Bgcn::MYC and either mel-Bam::HA or HA. Anti-HA immunoprecipitates were analyzed by Western blot. (C-D) IP experiment with sim-Bam and mel-Bgcn. (C) Cells were transfected with sim-Bam::HA and either mel-Bgcn::MYC or MYC. Anti-MYC immunoprecipitates were analyzed by Western blot. (D) Cells were transfected with mel-Bgcn::MYC and either sim-Bam::HA or HA. Anti-HA immunoprecipitates were analyzed by Western blot. Gels are loaded with 25% of total input (Input), 100% of immunoprecipitate (IP), and 10% of protein that did not immunoprecipitate (flow through, FT). (E-F) Ovaries of sim-bam-yfp;bam ⁻flies show a varying range of ovarian defects with mild (E) and moderate (F) examples shown for comparison. (G-H) Removal of a copy of bgcn (bgcn ¹) does not enhance the range of phenotypes seen in sim-bam-yfp;bam ⁻ovaries. No tumorous ovaries were seen (N > 50 ovarioles). (E-H) Ovaries are stained with antibodies to Vasa (green) and Hts-1B1 (red). Scale bar, 50μm.