Table 1. Nomenclature.
Nomenclature | Genotype | Description |
---|---|---|
D. melanogaster bam heterozygote | Females: bam Δ59/+ | D. melanogaster with only a single wildtype copy of bam. |
Males: bam BG/+ | ||
mel-bam-yfp; bam -, or sim-bam-yfp; bam - | Females: w; φ{w +, transgene}/+; bam Δ86/bam Δ59 (footnote a) | A single copy of a transgene in a D. melanogaster bam null mutant background. See Materials and Methods for description of different bam alleles. |
Males: w; φ{w +, transgene}/+; bam Δ86/bam BG | ||
2x mel-bam-yfp; bam -, or2x sim-bam-yfp; bam - | Females: w; φ{w +, transgene}, φ{w +, transgene}/+ +; bam Δ86/bam Δ59 | Two copies of a transgene in a D. melanogaster bam null background. |
2x sim-bam-yfp; bam -/+ | Females: w; φ{w +, transgene}, φ{w +, transgene}/+ +; bam Δ59/+ | Two copies of sim-bam-yfp in a D. melanogaster bam heterozygous background. These flies are siblings of 2x sim-bam-yfp; bam - flies. |
bam-α; bam - | Females: w; P{ry+, bam-α},bam Δ86 /bam Δ59 | A single copy of a bam transgene [23] in a bam null background. |
Males: w; P{ry+, bam-α},bam Δ86 /bam BG | ||
bam +wMel | w; bam BG/bam Δ59 +wMel | D. melanogaster bam hypomorph infected with Wolbachia strain wMel. |
bam-Tet | w; bam BG/bam Δ59 | D. melanogaster bam hypomorph without Wolbachia. |
a All experiments in females designated as mel-bam-yfp; bam - were performed with this genotype. As an additional control mel-bam-yfp; bam Δ86/bam BG (full genotype w; φ{w +, mel-bam-yfp}/+; bam Δ86/bam BG) was assayed for expression level in females in Fig 2A.