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HeLa cells expressing YFP tagged RalF proteins (green, described in Fig 2) were fixed and stained with Alexa Fluor 594 wheat germ agglutinin (WGA) to detect the plasma membrane (left) or anti-PDI antibody to detect the endoplasmic reticulum (right). DAPI (blue) is shown in the merged image. (Scale bar: 10 μm).
