Table 1. The Primers for Real-Time RT PCR.
| Gene | GenBank Accession Number | Primer sequences | Annealing temperature | Amplification efficiency | Product length |
|---|---|---|---|---|---|
| CCNB1 | XM_004016916 | Sense 5'-AACTAACTATGCTGGACTACGA-3' | 52°C | 98.8% | 146 bp |
| Anti-sense 5'-CAGGGATTCTTCAGTGTATG-3' | |||||
| CCNG2 | XM_004009930 | Sense 5'-TCGGATTGTTGAACCTCT-3' | 48°C | 92.0% | 134 bp |
| Anti-sense 5'-ATCTTCGACTTTGGCATT-3' | |||||
| TP53 | NM_001009403 | Sense 5'-CCACCATCCACTACAACTTCA-3' | 55°C | 101.2% | 147 bp |
| Anti-sense 5'-CAGGACAGGCACAAACACG-3' | |||||
| TNFRSF17 | XM_004020779 | Sense 5'-TAACGCAGACCTGGATGT-3' | 48°C | 91.8% | 214 bp |
| Anti-sense 5'-CAGACTGTCGCAGTAGCC-3' | |||||
| GAPDH | NM_001190390 | Sense 5'-ACGTGTCCGTTGTGGATC-3' | 52°C | 96.7% | 158 bp |
| Anti-sense 5'-GTGAGTGTCGCTGTTGAAGT-3' |