. 2015 Aug 20;10(8):e0135344. doi: 10.1371/journal.pone.0135344

Table 3. The Developmental Ability of Cloned Embryos Derived from Differentially Cultured Fibroblasts.

Treatments	No. cultured embryos	2-cell	4-cell	8-cell	morula	blastocyst^**
Control ^*	145	65 (44.8%) ^a	48 (33.1%) ^a	37 (25.5%) ^a	28 (19.3%) ^a	9 (6.2%) ^a
Full confluency	132	68 (51.5%) ^a	55 (41.7%) ^a ^b	46 (34.8%) ^a ^b	36 (27.3%) ^a ^b	13 (9.8%) ^a ^b
Serum starvation	101	57 (56.4%) ^a ^b	47 (46.5%) ^b	41 (40.6%) ^b	34 (33.7%) ^b	12 (11.9%) ^a ^b
Full confluency+serum starvation	127	82 (64.6%) ^b	68 (53.5%) ^b	58 (45.7%) ^b	49 (38.6%) ^b	22 (17.3%) ^b

^{a, b}Values with different superscripts within the same column are significantly different (P<0.05).

*Control: fibroblasts were cultured to 70–80% confluency.

**The data include all blastocysts from 12 days of culture.

Data were collected from 10 series of SCNT experiments.