Table 2. Mean values (± STD) for contents of total fatty acids and lipid fractions as well as unsaturation indices (UI) of the PLFA fraction in untreated zebrafish eggs and eggs treated with 2,4-DNP at 24, 30 and 48 hpf.

Values for 2,4-DNP treated eggs that were statistically significantly different from those for untreated eggs at the respective stage are marked with * (p < 0.05; Student’s t-test). Note that values for untreated eggs correspond to respective data presented in Fig 2 and are listed here for comparison. n = 3–4, with 50 eggs per n; hpf: hours post fertilization.

	total fatty acids (mg g -1 dw)		PFLAs (mg g -1 dw)		glycolipids (mg g -1 dw)		neutral lipids (mg g -1 dw)		PFLA UI
	untreated	2,4-DNP	untreated	2,4-DNP	untreated	2,4-DNP	untreated	2,4-DNP	untreated	2,4-DNP
24 hpf	62.5 ± 4.9	50.4 ± 11.6	45.2 ± 5.2	38.7 ± 4.9	2.8 ± 0.2	1.0 ± 0.5*	14.5 ± 0.6	14.3 ± 2.4	1.91 ± 0.02	1.48 ± 0.02*
30 hpf	62.6 ± 9.0	47.8 ± 3.7*	47.8 ± 8.7	33.5 ± 1.0*	1.8 ± 0.3	0.7 ± 0.1*	12.9 ± 0.6	13.7 ± 2.7	1.85 ± 0.01	1.44 ± 0.03*
48 hpf	66.2 ± 7.4	51.6 ± 7.0*	49.9 ± 6.1	32.6 ± 11.7*	2.3 ± 0.7	0.9 ± 0.5*	14.1 ± 3.4	18.0 ± 7.6	1.89 ± 0.04	1.76 ± 0.05*