Table 3. SBV RNA quantity detected in Culex pipiens from three colony lines fed a mixture of blood and tissue culture passaged virus or intrathoracically (IT) inoculated and then incubated for 14 days prior to processing.
Cq | Culex pipiens colony line / Treatment | |||
---|---|---|---|---|
Brookwood (Membrane fed) | Caldbeck (Membrane fed) | Wageningen (Membrane fed) | Brookwood (IT inoculated) | |
≥40 | 92 | 71 | 113 | 0 |
40–35 | 9 | 3 | 6 | 0 |
35–30 | 7 | 6 | 3 | 1 |
30–25 | 4 | 3 | 1 | 0 |
25–20 | 2 | 2 | 0 | 5 |
20–15 | 0 | 0 | 0 | 29 |
Total | 114 | 85 | 123 | 35 |
Median C q | 33.3 | 32.6 | 35.4 | 17.3 |
Range C q | 39–23.3 | 37.7–22.6 | 40–28.8 | 30–15.7 |
RNA detection rate (%) | 19.3 | 16.5 | 8.1 | 100 |