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. 2015 Aug 20;11(8):e1005446. doi: 10.1371/journal.pgen.1005446

Fig 2. The evIs129[emb-9p::unc-5] phase 3 A/P polarity reversals are dependent on unc-5 and unc-40, but independent of unc-6/netrin.

Fig 2

(A) Quantification of phase 3 A/P polarity reversals in evIs98C (a multi-copy transgenic array of unc-5(+) regulated by its own promoter) or evIs129 (another multi-copy transgenic array of unc-5(+) driven by the emb-9 promoter) versus evIs129 treated with unc-5(RNAi) or in the background of unc-40 or unc-6 null alleles. Bars represent the percentage of posterior DTC phase 3 polarity reversals. The corresponding raw data are presented in S2 Table. Error bars indicate standard error of the sample proportion. Comparisons of the phase 3 A/P reversals were made between each corresponding pair as indicated by the connecting lines. ***P <0.00001; ns = not significant (P≥0.01). (B) Quantification of phase 2 D/V precocious migrations in the strains presented in panel (A). Bars represent the percentage of posterior DTCs that migrate precociously to the dorsal side as determined by the shape of the gonad arms. The corresponding raw data are presented in S2 Table. Error bars indicate standard error of the sample proportion. Comparisons of the D/V precocious migration were made between each corresponding pair as indicated by the connecting lines. ***P <0.00001; ns = not significant (P≥0.01). The corresponding control for RNAi was grown on empty vector RNAi feeding bacteria.