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. 2015 Aug 21;10(8):e0135245. doi: 10.1371/journal.pone.0135245

Fig 3. mTORC1/2 dual inhibition blocks VEGF-mediated angiogenesis.

Fig 3

HUVEC-coated Cytodex beads were embedded in fibrin gels as in Fig 2, then the EC were stimulated with 50 ng/ml VEGF, and were treated with PP242 or carrier as indicated. A) Representative images of EC sprouts after 18 hours incubation. B) Quantitation of the number of sprouts per bead. C) Quantitation of the length of the sprouts (n = 3 independent experiments, *P<0.05 by ANOVA, scale bar = 95 um). D) Collagen gel onplants containing VEGF (100 ng/onplant) and PP242 or carrier, were placed on chicken embryo CAM as described in Methods. Quantitation of neovascularization after 64 hours of exposure to VEGF supplemented with 1, 5, 10 or 50 uM PP242 (n > 48 onplants or 16 chicken embryos per group, *P<0.05 by ANOVA).