Fig. 3.

Sensitivity of ΔuvrA and ΔsigRrsrA mutants to UV and thiol-reactive oxidants and electrophiles. Serially diluted spores of wild-type (WT), ΔsigRrsrA, ΔuvrA strains were spotted on NA plates with or without added chemicals such as diamide (0.6 mM), methyl hydroquinone (MHQ, 1 mM) and methyl glyoxal (MG, 1 mM). For UV irradiation, the spotted plates were exposed to UV light from UV cross-linker for 30 seconds. Plates were incubated at 30°C for 1 (untreated), 2 (UV, diamide), 5 (MHQ) or 6 days (MG).