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. 2015 Mar 26;6(16):14179–14190. doi: 10.18632/oncotarget.3651

Figure 5. Recruited mast cells enhance PCa cell invasion via alteration of AR-mediated CD133 stem/progenitor cell population.

Figure 5

A. Co-culture of mast cells with PCa cells led to increased stem cell marker proteins, CD133, NANOG, and CXCR4. B. Flow cytometry analysis showed increased CD133+ cell population in PCa after being co-cultured with HMC-1 cells. C. Sphere formation assay validated the increased stem/progenitor cells. The PCa cells were co-cultured with or without HMC-1 cells for 5 days. PCa cells were then mixed with matrigel (1:1, v/v), plated in 24-well plates, and cultured for 7 days. Quantification results are presented in the lower panel. D. Knockdown of AR in PCa cells changes their responsiveness to mast cells-promoted CD133+ stem cell marker expression. * p < 0.05.