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. 2015 Mar 29;6(16):14260–14273. doi: 10.18632/oncotarget.3680

Figure 1. In vitro agent validation.

Figure 1

A) Flow cytometer analyses show high uPAR expression on HT-29 cells while no expression is detectable on the Caco-2 cell line. B) Graph shows the serum stability of hybrid ATN-658. An increase in aggregates and albumin bound agents is seen over time, with 60% of the agent still free after 48 h. C) Cell based plate assay analyses show the specific binding of hybrid ATN-658 on uPAR expressing HT-29 cells. Hybrid ATN-658 signal intensities differed significantly from the control hybrid MOPC-21 at all dose groups except 0 nM. D) No specific binding on the control cell line Caco-2 and there were no significant differences between both tracers at all dose groups. A.U.= arbitrary units.