Skip to main content
. 2015 Apr 29;6(16):14344–14359. doi: 10.18632/oncotarget.3852

Figure 4. Inhibition of gastric cancer cell migration and invasion by AIB1 down-regulation.

Figure 4

A. Cells transfected with si-AIB1-709 or si-NC were starved overnight and then seeded in the transwell chambers without matrigel for migration assay, and coated with matrigel for invasion assay, respectively. After a 24 h-culture, non-migrating (or non-invading) cells in the upper chamber were removed and migrating (or invading) cells were stained and calculated in five microscopic fields per sample. Shown are representative images of migrating (or invading) cells (left panels). Histograms (right panels), corresponding to left panels, show means ± SE of the numbers of migrating (or invading) cells from three independent assays. ***P < 0.001. B. qRT-PCR assay was performed to investigate the effect of AIB1 knockdown on the expression of metastasis-related genes MMP-2, -7, -9 and -14 in gastric cancer cells. Expression levels of these genes were normalized with 18S rRNA levels. Data were presented as mean ± SE. *P < 0.05; **P < 0.01; ***P < 0.001.