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. 2015 May 2;6(16):14385–14398. doi: 10.18632/oncotarget.3775

Figure 1. Efffects of 5-FU, NVP-BEZ235 or their combination on cell viability and apoptosis.

Figure 1

A–D. Cells viability was analyzed using Cell Counting Kit-8 at 0, 3, 6, 12 and 24 hours after (A) 50, 100, 200, or 400 uM 5-FU treatment, (B) 100, 200, 400, or 800 nM NVP-BEZ235 treatment, (C) the combination treatment of 200 uM 5-FU and 400 nM NVP-BEZ235 in HCT-116 cells, (D) Cell viability was detected in RKO cells after 24 hours combination treatment. E. HCT-116 cells apoptosis was analyzed using FACS technique at 12 hours after the treatment of 200 uM 5-FU, or 400 nM NVP-BEZ235, or their combination. F. The percentage of apoptotic cells were used to calculate. Data represent the mean ± S.D. of four independent experiments.