Figure 7. The antitumor effect of combination treatment in vivo.
A–C. Nude mice were injected s.c. with 1 × 106 HCT-116 cells. Once the tumor was measurable, mice were treated daily with 5-FU at 25 mg/kg by i.p. injection, or 40 mg/kg NVP-BEZ235 by oral gavage, or their combination for 15 consecutive days. (A) Representative tumors at the end of the experiment, (B) tumors weight, (C) tumor volume at indicated time points after treatment was calculated. Data represent the mean ± S.D. of four independent experiments. D. Paraffin-embedded sections of control or treated tumor tissues from mice were analyzed by P-Akt(S473), ki67 and active Caspase-3 IHC staining. E. and F. Quantitative analysis of ki67 and active Caspase-3 staining corresponding to the images in D. Data represent the mean ± S.D. of four independent experiments.