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. 2015 Mar 30;6(16):14488–14496. doi: 10.18632/oncotarget.3697

Figure 1.

Figure 1

A, the 400X photographs of LNCaP and LNCaP-AI. LNCaP-AI cells exhibited a neuronal morphology with compactly rounded cell bodies, having extended and fine-branched processes. B, COL6A1 is upregulated in induced castration-resistant LNCaP cells. The proteins were harvested at 0 day, 4 days, 2 weeks, 1 month, 1.5 months, and 2 months after induction of SR conditions. The expression of COL6A1, AR, Akt, and p-Akt were examined by western blot. COL6A1 and AR and Akt were upregulated during castration. However, p-Akt level was stable or even decreased during castration. C, COL6A1 is overexpressed in patients with CRPC. In Masson's trichrome staining, collagen stains blue and cytoplasm stains red. Thus, collagen-rich tumor stroma (blue) can be differentiated from normal stroma (red, because preexisting normal host stroma is rich in smooth muscle bundles). The picture of H&E, Masson's trichrome stain, and IHC of COL6A1 of paired androgen-naïve and patients with CRPC were photographed in 200X. The reactive stroma and COL6A1 expression were significantly upregulated in patients with CRPC.