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. 2015 Mar 30;6(16):14488–14496. doi: 10.18632/oncotarget.3697

Figure 2. COL6A1 could decrease cell proliferation in prostate cancer cells.

Figure 2

A, All vectors used in this study are shown above. COL6A1 overexpression constructs were inserted after CMV promoter of pCDH-CMV-MCS-EF1-Puro (CD510B-1) or pCDH-CMV-MCS-EF1-copGFP (CD511B-1). pLKO.1 TRC cloning vector (Addgene Plasmid 10878) was employed to generate shRNA constructs against COL6A1. B, The COL6A1 expressions of all stable cell lines was examined by western blot. COL6A1 was successfully knocked down in PC-3 cells and was successfully overexpressed in LNCaP cells. C, CCK-8 assay was used to detect the proliferation capability of stable transfected cell lines. D, E, The cell cycle alteration of COL6A1 knock-down or overexpression in stable cell lines was detected by flow cytometry.