Figure 4. TGFβ-I mediated c-Myc expression is regulated through NFAT-1.

A. FUMMT-1 and CS-99 cells were starved, treated with TGFβ-I (5 ng/ml) for 24h, lysed and immunoblotted for c-Myc and GAPDH. B. Effects of TGFβ-I and LY2157299 on NFAT-1 translocation was assayed by immunofluorescence in FUMMT-1. The images were acquired using a 40X objective (1.6X Optovar) with Zeiss Observer 2. Scale bar represents 50 um. C. FUMMT-1 cells were starved, pretreated with NFAT-1 inhibitor (100 μM, 1 h) subsequently treated with TGFβ-I (5 ng/ml) for 24 h, lysed and processed for Western blotting.