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. 2015 Mar 30;6(16):14646–14655. doi: 10.18632/oncotarget.3711

Figure 5. TGFβ-I induced proliferation and cell cycle is mediated through c-Myc.

Figure 5

A. FUMMT-1 cells were starved pretreated with c-Myc inhibitor subsequently treated with Vehicle (V) or TGFβ-I (5 ng/ml) and relative cell viability was quantified using MTS assay. B. FUMMT-1 cells were transfected with either non-target si-RNA (Si-CTL) or Si-c-Myc, cells were platted in 96 well plate and TGFβ-I induced proliferation was evaluated using MTS assay. Efficiency of c-Myc silencing is shown in inset. C. FUMMT-1 cells were starved pretreated with c-Myc inhibitor (10058-F4, 5 μM, 1 h) and treated with Vehicle (V) or TGFβ-I (5ng/ml) for 24 h lysed and processed for Western blotting. D. FUMMT-1 cell were starved pretreated with LY2157299 for 1 h, subsequently treated with TGFβ-I for 24 h, lysed and processed for Western blotting. E. RNA was isolated from UCS patient samples and relative c-Myc mRNA levels with respect to non-recurrent patient sample having highest ΔCT value were quantified using RT-qPCR, Error bars represent standard error of mean.*, P < 0.05 was considered significant. Error bars represent SD except where indicated.